Pcr Template Concentration - Web optimal concentration depends on template, buffer, dna and dntps (each has the potential to chelate magnesium) if [mg 2+]. Web the following guidelines are provided to ensure successful pcr using neb's taq dna polymerase. Web a number of factors can affect the amount of free mg 2+ in a reaction, including dna template concentration, chelating agents.
Web a number of factors can affect the amount of free mg 2+ in a reaction, including dna template concentration, chelating agents. Web optimal concentration depends on template, buffer, dna and dntps (each has the potential to chelate magnesium) if [mg 2+]. Web the following guidelines are provided to ensure successful pcr using neb's taq dna polymerase.